Coding

Part:BBa_K3468081:Design

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-20)


PETase T266P


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Reading the literature found that proline can be introduced into the flexible loop to improve the thermal stability of the protein. T266P is located in the loop region of the protein.

Fig.1 Thr266 in PyMOL
Fig.2 T266P in PyMOL

Source

Ideonalla sakaiensis

References